MEDICAL DEVICE LATEST RESEARCH

|Biomedical 2019|

|Latest Research on Medical device|

|Extracting Exosomes to Detect Signs of Cancer in Urine|

A new reserach  has been invented to detect signs of cancer in urine

A new device uses anchored nanowires to capture extracellular vesicles from pee for microRNA analysis.

Exosomes are tiny membrane-bound packages that are released from practically every cell type and found in a wide range of body fluids. Containing RNAs, proteins, and other cell components, they are believed to be involved in communication between cells, and there’s evidence that their abundance and content may change with disease state. Consequently, there is a growing interest in collecting and analyzing these vesicles for diagnostic purposes. Researchers who are interested in the diagnostic potential of microRNAs, for example, are especially keen to collect exosomes because the RNAs they contain degrade more slowly than free-floating RNAs.

The nanowires are approximately 100 nm wide and 2,000 nm long, and are held in place in a microfluidic chamber by a silicon-based organic polymer. The wires create a large, positively charged surface area, says Yasui, which the group hypothesized would be “a powerful tool” for collecting negatively charged exosomeshey were right. Passing just one ml of urine through the device, followed by one ml of lysis buffer, enabled the team to collect and sequence small RNAs that yielded approximately threefold more microRNA species than the amount obtained by ultracentrifugation of 20 times more urine.

The team went on to analyze exosomes from healthy human subjects and patients with various cancers, and found differences in microRNA profiles

 |SOURCES : Sci Adv, 3:e1701133, 2017) |

https://biomedicine.conferenceseries.com/

|March 2019 29-30 

Hong Kong|